Bifalgorithm post · Astronomy · and figure 3.25). Keep in mind that these names—even though they are Latinized—refer to locations on the

How are tineas diagnosed in the laboratory?

How are tineas diagnosed in the laboratory? (Astronomy · and figure 3.25). Keep in mind that these names—even though they are Latinized—refer to locations on t…

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How are tineas diagnosed in the laboratory?

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"Several approaches may be used to diagnose tineas. A Wood’s lamp (also called a black lamp) with a wavelength of 365 nm is often used. When directed on a tinea, the ultraviolet light emitted from the Wood’s lamp causes the fungal elements (spores and hyphae) to fluoresce. Direct microscopic evaluation of specimens from skin scrapings, hair, or nails can also be used to detect fungi. Generally, these specimens are prepared in a wet mount using a potassium hydroxide solution (10%–20% aqueous KOH), which dissolves the keratin in hair, nails, and skin cells to allow for visualization of the hyphae and fungal spores. The specimens may be grown on Sabouraud dextrose CC (chloramphenicol/cycloheximide), a selective agar that supports dermatophyte growth while inhibiting the growth of bacteria and saprophytic fungi. To diagnose tineas, the dermatophytes may be grown on a Sabouraud dextrose CC agar plate. This culture contains a strain of Trichophyton rubrum, one of the most common causes of tineas on various parts of the body."

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