Chapter

ARTICLE IN PRESS

From Covid research by Sambif

Concepts

1. Cell Cloning

   "showed strong plasma ADCC activity ( Fig. 1b ) to conduct mAb cloning. Memory B cells recognizing WT S protein (CD19 + CD27 + IgG + Spike + ) were sorted individually ( Fig. 3b ) for B cell receptor

2. ADCC Activity

   "To identify which mAbs possess ADCC activity, we conducted the ADCC assay using NK - 92 MI FcR cells and target cells, including WT, Delta, or BA.4/5 S protein overexpressing 293T cells as previously

3. Antibody Binding

   "Pseudotyped virus neutralization assays indicated that only 5SB12 and 1 - 2SA8 can neutralize a diverse panel of pseudotyped virus - expressing S protein variants with an IC 50 below the nanomolar to