Cell Cloning

"showed strong plasma ADCC activity ( Fig. 1b ) to conduct mAb cloning. Memory B cells recognizing WT S protein (CD19 + CD27 + IgG + Spike + ) were sorted individually ( Fig. 3b ) for B cell receptor cloning. The ELISA results revealed that several resultant mAbs bind strongly with WT S prote in with EC 50 lower than sub - nM levels ( Fig. 3c and Supplementary Fig. 3a ). We used different domains of the S protein to identify the binding region of these mAbs ( Supplementary Fig. 3b ). The results revealed that they do not restrict binding to certain subunits of WT S protein, such as NTD, RBD, and S2 regions ( Fig. 3c and Supplementary Fig. 3b ). Several of these mAbs also showed cross - recognition activity ( Fig. 3c )."